PL Quality Assessment Protocol
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Gross examination: (in white bowl)
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1. Size for PL12 P. vannamei
(>8mm, <300/g
about 0.003g)
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2. Size variation (big and small)
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3. Benthic behaviour
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4. Swimming activity
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5. Colour (red/black/brown)
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Microscopic exam: (sample of 20-30 PL – scoring system)
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1. HP (Full/dark, lipid vaculoles 10, if not 5 or 0)
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2. Gut (Full, vacuoles, peristalsis 10, 5, 0)
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3. Fouling (None 10, medium 5, heavy 0)
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4. Deformity/necrosis (None 10, medium 5, heavy 0)
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5. MGR in 6th Segment (>75% 10, 50-75% 5, <50% 0)
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6. MBV by Malachite in HP (None 10, few 5, lots 0)
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Scoring: Max 60, pass 50, if < 50 or more than 3 zeros: fail.
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荧光弧菌
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貌似有点恐怖啊
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good MRG(肌肠比?)
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poor MRG
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脂肪滴
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