PL Quality Assessment Protocol（虾苗质量评估）

冰山

哈哈，首次使用电脑登录水产频道发个帖子纪念！ （老外的东西，本人英语不好，大家自己翻译吧！）

PL Quality Assessment Protocol

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Gross examination: (in white bowl)

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1.   Size for PL12 P. vannamei
(>8mm, <300/g
about 0.003g)

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2.   Size variation (big and small)

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3.   Benthic behaviour

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4.   Swimming activity

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5.   Colour (red/black/brown)

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Microscopic exam: (sample of 20-30 PL – scoring system)

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1.   HP (Full/dark, lipid vaculoles 10, if not 5 or 0)

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2.   Gut (Full, vacuoles, peristalsis 10, 5, 0)

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3.   Fouling (None 10, medium 5, heavy 0)

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4.   Deformity/necrosis (None 10, medium 5, heavy 0)

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5.   MGR in 6th Segment (>75% 10, 50-75% 5, <50% 0)

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6.   MBV by Malachite in HP (None 10, few 5, lots 0)

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Scoring: Max 60, pass 50, if < 50 or more than 3 zeros: fail.

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&nbsp;Salinity stress test (300 PL at PL 10-12)
Either: transfer to freshwater for 30 minutes and then back to seawater for 30  minutes >75% OK
Or: 1:1 mix with freshwater and after 2 hours >90% OK

Vibrio bacteria examination
100 PL from tank, sterilize in alcohol, washed, ground up
Streaked by 1 sterile wire loop over TCBS and TSA plates
Incubate at 30-35oC for 18-24 hours
Green colonies <30/plate OK (preferably 0)
Yellow colonies <80/plate OK
No Luminescence on TCBS/TSA OK

PCR testing (for WSSV)
150 PL (preferably weakest shrimp)
Preserve in 70-95% alcohol or ship alive
Use 2-step, nested PCR test
If positive result – reject batch.

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PL Nutrition
Make sure PL that are bought have been well fed, and have received plenty of artemia, HUFAs (esp. EPA, DHA and AA), Lecithin (1%), Cholesterol (1%) Choline chloride (0.1%), Vitamin C (1-3% AAP), Vitamin E (0.01%), Astaxanthin (0.01%) and probiotics (not antibiotics)
Such additions improve stress - resistance and grow-out production

冰山

回复 5# 浪迹天涯


    呵呵，这些标准看似很简单，要都做到这些标准就不简单了！

冰山

回复 7# 大虾熊


    呵呵，太依赖感觉和经验容易掉经验的陷阱，被表现迷惑！

冰山

回复 10# 原来鱼旺


    这个你需要找谷歌帮忙下，我也是找谷哥的。