n
Gross examination: (in white bowl)
n
1. Size for PL12 P. vannamei
(>8mm, <300/g
about 0.003g)
n
2. Size variation (big and small)
n
3. Benthic behaviour
n
4. Swimming activity
n
5. Colour (red/black/brown)
n
Microscopic exam: (sample of 20-30 PL – scoring system)
n
1. HP (Full/dark, lipid vaculoles 10, if not 5 or 0)
n
2. Gut (Full, vacuoles, peristalsis 10, 5, 0)
n
3. Fouling (None 10, medium 5, heavy 0)
n
4. Deformity/necrosis (None 10, medium 5, heavy 0)
n
5. MGR in 6th Segment (>75% 10, 50-75% 5, <50% 0)
n
6. MBV by Malachite in HP (None 10, few 5, lots 0)
n
Scoring: Max 60, pass 50, if < 50 or more than 3 zeros: fail.