求大神翻译

上帝的爱

A microsporidian parasite, Enterocytozoon hepatopenaei (abbreviated as EHP), is an emerging pathogen for penaeid shrimp. EHP has been found in several shrimp farming countries in Asia including Vietnam, Thailand, Malaysia, Indonesia and China, and is reported to be associated with growth retardation in farmed shrimp. We examined the histological features from infected shrimp collected from Vietnam and Brunei, these include the presence of basophilic inclusions in the hepatopancreas tubule epithelial cells, in which EHP is found at various developmental stages, ranging from plasmodia to mature spores. By a PCR targeting the 18S rRNA gene, a 1.1聽kb 18S rRNA gene fragment of EHP was amplified, and this sequence showed a 100% identity to EHP found in Thailand and China. This fragment was cloned and labeled with digoxigenin-11-dUTP, and in situ hybridized to tissue sections of infected Penaeus vannamei (from Vietnam) and P. stylirostris (Brunei). The results of in situ hybridization were specific, the probe only reacted to the EHP within the cytoplasmic inclusions, not to a Pleistophora -like microsporidium that is associated with cotton shrimp disease. Subsequently, we developed a PCR assay from this 18S rRNA gene region, this PCR is shown to be specific to EHP, did not react to 2 other parasitic pathogens, an amoeba and the cotton shrimp disease microsporidium, nor to genomic DNA of various crustaceans including polychaetes, squids, crabs and krill. EHP was detected, through PCR, in hepatopancreatic tissue, feces and water sampled from infected shrimp tanks, and in some samples of Artemia biomass.

上帝的爱

一个个拆开我还可以读出来，有关孢子虫的，大家帮组一下，目前正在研究这个问题

狼牙166375

一种微孢子寄生虫-对虾肠孢子虫（缩写为EHP），是一种针对对虾的新兴病原体，目前已在亚洲包括越南、泰国、马来西亚、印度尼西亚和中国的几个虾养殖国家发现，报道称该种病原能导致虾生长迟缓。我们检查了收集自越南和文莱感染虾体的组织特征，这其中就包含了嗜碱性夹杂物的肝曲小管上皮细胞，在这里发现了EHP从疟原虫到成熟孢子的多个发育阶段，通过PCR特异性扩增该EHP的18SrRNA基因，一条长为1.1kbp的EHP18SrRNA基因片段被扩增出来，且该片段的基因序列与在泰国和中国发现的EHP序列显示出100%的同源性。该基因片段被克隆后被标上地高辛-11-dUTP标记，然后原位杂交到南美白对虾（来自越南）和南美白细脚对虾（文莱）的感染组织部分，原位杂交的结果是特异性的，探针只在EHP的细胞质内含物中发生了反应，而不是一种与棉花虾疾病相关的具褶孢虫属的孢子虫。随后，我们又针对这个EHP的18SrRNA基因区域进行了PCR检测，其结果显示对EHP是特异性的,并不与其他两种孢子虫病原发生反应，阿米巴和棉花虾疾病微孢子虫，也不是其他甲壳类包括多毛类、鱿鱼、螃蟹和磷虾的基因组DNA，通过PCR我们在感染虾塘的虾肝脏组织、粪便、水样和一些卤虫的样品中发现了EHP。

一片云

狼牙166375 发表于 2015-12-14 10:46
一种微孢子寄生虫-对虾肠孢子虫（缩写为EHP），是一种针对对虾的新兴病原体，目前已在亚洲包括越南、泰国、 ...

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上帝的爱

狼牙166375 发表于 2015-12-14 10:46
一种微孢子寄生虫-对虾肠孢子虫（缩写为EHP），是一种针对对虾的新兴病原体，目前已在亚洲包括越南、泰国、 ...

谢谢狼牙老板，这个月开始系统研究和处理对虾亲虾防控肠孢子虫