原文作者: , , , , .
原文摘要:Shrimp (Penaeus monodon and P. vannamei) specimens were submitted to the University of Arizona's Aquaculture Pathology Laboratory (UAZAPL) and to the Texas Veterinary Medical Diagnostic Laboratory (TVMDL) in 2007 and 2008 from shrimp-rearing facilities in India and Indonesia for histological testing. These were found to present prominent golden to greenish-brown needle- and plate-like birefringent crystals within multifocal hemocytic granulomas in the antennal gland tubules and peritubular hemal sinuses. Their appearance was very similar to melamine-cyanuric acid-induced crystals previously described from cat and dog kidneys with melamine-associated renal failure (MARF). Significant chronic mortalities were reported from the affected P. vannamei farms in Indonesia, but were not observed in the affected P. monodon facility in India. Shrimp feed was suspected as the source of melamine due to the similarity of the shrimp antennal gland lesions to those present in MARF. 'Normal' and 'suspect' feed samples from the facilities in Indonesia and India were sent to regional laboratories for analysis. Melamine was detected in 2 of 4 feed samples from an affected Indonesian farm. Melamine was not detected in 'normal' feed from the Indian facility, but it was found in 2 'suspect' samples (Feeds A and B) at levels of 183.39 and 112.50 ppm, respectively. A bioassay of Feed A with P. vannamei at UAZAPL confirmed that the melamine-contaminated feed induced prominent granulomas in the antennal gland with the characteristic crystals within 10 d of the first feeding, experimentally confirming the direct relationship of melamine-adulterated feed to the unique pathology observed.
印度和印尼的对虾(南美白和斑节)标本2007年和2008年分别送到亚历山大和德克萨斯,在触角腺和血窦周围发现了针状棕绿色晶体物。这种物体非常类似三聚氰胺晶体,因为之前有报道美国的猫和狗的肾脏衰竭鱼这种物质有关。印尼的报告上指出对虾的慢性死亡症状,却找不出病因。(这个像不像我们说的偷死?),在他们的饲料中发现了“疑似”物质,含量分别为183.39和112.50ppm,最后并予以确认。实验室中感染三聚氰胺的对虾表现出虾触角腺肿大 的症状。亚历山大实验室最后确定是饲料掺进三聚氰胺导致的问题。
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